: Determine the cycle at which the next by-product of fluorescence with regard to cycle amount is maximal (Rasmussen, 2001). Area fitting into a parabola may be used to outline the height place with sub‐cycle precision. 10 Commercially accessible RNA extraction kits like the Qiagen RNeasy® Mini Plant package or https://www.facebook.com/NewTechFind/posts/pfbid02bCsrCGJtTg2r1aCQEoachZh9MmkmeiEeXcTXZBDji5BdLR4ZWcPQtKHvAsjvNUEBl