A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of the whole right and partial left ends of the novel FAdV-4 genome. which worked through cell-free reactions of restriction digestion and Gibson assembly. Three recombinant viruses were constructe... https://www.nacrack.com/quick-grab-Chainsaw-File-super-grab/